immunoassay elisa kits Search Results


elisa analytical kit for bevacizumab  (United Immunoassay)
90
United Immunoassay elisa analytical kit for bevacizumab
Monomeric <t>bevacizumab</t> significantly decreases when phosphate buffered saline (PBS) is used as a diluent. Representative SE-HPLC chromatograms showing bevacizumab in PBS at time 0 and after 3 h and graphical representation of SE-HPLC area and the slope for bevacizumab diluted with PBS at time 0 versus 3 h at room temperature ( a ), graphical representation of SE-HPLC area and the slope of the effect of dilution on monomer concentration of ranibizumab over time at 37°C in the manufacturer’s formulation; formula 1 ( b ). Shown are time 0 and after 24 h storage at 37°C.
Elisa Analytical Kit For Bevacizumab, supplied by United Immunoassay, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/bevacizumab+elisa+kits+ava+e+u51/pmc05830485-85-0-14
Average 90 stars, based on 1 article reviews
elisa analytical kit for bevacizumab - by Bioz Stars, 2026-10
90/100 stars
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elisa kits advia centaur xp immunoassay system  (Siemens AG)
90
Siemens AG elisa kits advia centaur xp immunoassay system
Monomeric <t>bevacizumab</t> significantly decreases when phosphate buffered saline (PBS) is used as a diluent. Representative SE-HPLC chromatograms showing bevacizumab in PBS at time 0 and after 3 h and graphical representation of SE-HPLC area and the slope for bevacizumab diluted with PBS at time 0 versus 3 h at room temperature ( a ), graphical representation of SE-HPLC area and the slope of the effect of dilution on monomer concentration of ranibizumab over time at 37°C in the manufacturer’s formulation; formula 1 ( b ). Shown are time 0 and after 24 h storage at 37°C.
Elisa Kits Advia Centaur Xp Immunoassay System, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/elisa+kits+advia+centaur+xp+immunoassay+system/pm28425257-39-18-25
Average 90 stars, based on 1 article reviews
elisa kits advia centaur xp immunoassay system - by Bioz Stars, 2026-10
90/100 stars
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tacrolimus (tac) enzyme-linked immunoassay (elisa) kits  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology tacrolimus (tac) enzyme-linked immunoassay (elisa) kits
Monomeric <t>bevacizumab</t> significantly decreases when phosphate buffered saline (PBS) is used as a diluent. Representative SE-HPLC chromatograms showing bevacizumab in PBS at time 0 and after 3 h and graphical representation of SE-HPLC area and the slope for bevacizumab diluted with PBS at time 0 versus 3 h at room temperature ( a ), graphical representation of SE-HPLC area and the slope of the effect of dilution on monomer concentration of ranibizumab over time at 37°C in the manufacturer’s formulation; formula 1 ( b ). Shown are time 0 and after 24 h storage at 37°C.
Tacrolimus (Tac) Enzyme Linked Immunoassay (Elisa) Kits, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/tacrolimus++tac++enzyme+linked+immunoassay++elisa++kits/pm40650653-40-0-8
Average 90 stars, based on 1 article reviews
tacrolimus (tac) enzyme-linked immunoassay (elisa) kits - by Bioz Stars, 2026-10
90/100 stars
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enzyme-immunoassay (elisa) kits for testosterone, dhea and estradiol  (Salimetrics LLC)
90
Salimetrics LLC enzyme-immunoassay (elisa) kits for testosterone, dhea and estradiol
Monomeric <t>bevacizumab</t> significantly decreases when phosphate buffered saline (PBS) is used as a diluent. Representative SE-HPLC chromatograms showing bevacizumab in PBS at time 0 and after 3 h and graphical representation of SE-HPLC area and the slope for bevacizumab diluted with PBS at time 0 versus 3 h at room temperature ( a ), graphical representation of SE-HPLC area and the slope of the effect of dilution on monomer concentration of ranibizumab over time at 37°C in the manufacturer’s formulation; formula 1 ( b ). Shown are time 0 and after 24 h storage at 37°C.
Enzyme Immunoassay (Elisa) Kits For Testosterone, Dhea And Estradiol, supplied by Salimetrics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/enzyme+immunoassay++elisa++kits+for+testosterone++dhea+and+estradiol/ppr0332561-128-18-13
Average 90 stars, based on 1 article reviews
enzyme-immunoassay (elisa) kits for testosterone, dhea and estradiol - by Bioz Stars, 2026-10
90/100 stars
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tgf-β1 emax immunoassay system elisa kit  (Promega)
90
Promega tgf-β1 emax immunoassay system elisa kit
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Tgf β1 Emax Immunoassay System Elisa Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/elisa+kits+tgf+%CE%B2+1+emax+immunoassay+system/pmc02193974-113-29-28
Average 90 stars, based on 1 article reviews
tgf-β1 emax immunoassay system elisa kit - by Bioz Stars, 2026-10
90/100 stars
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nrf2 enzyme linked immunoassay (elisa) kits  (Glory Science)
90
Glory Science nrf2 enzyme linked immunoassay (elisa) kits
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Nrf2 Enzyme Linked Immunoassay (Elisa) Kits, supplied by Glory Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/nrf2+enzyme+linked+immunoassay++elisa++kits/pmc08099916-65-6-18
Average 90 stars, based on 1 article reviews
nrf2 enzyme linked immunoassay (elisa) kits - by Bioz Stars, 2026-10
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elisa enzyme immunoassay kits  (Wuhan EIAab Science)
90
Wuhan EIAab Science elisa enzyme immunoassay kits
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Elisa Enzyme Immunoassay Kits, supplied by Wuhan EIAab Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/elisa+enzyme+immunoassay+kits/pm37755286-57-8-14
Average 90 stars, based on 1 article reviews
elisa enzyme immunoassay kits - by Bioz Stars, 2026-10
90/100 stars
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enzyme immunoassay/ elisa kits  (Abnova)
90
Abnova enzyme immunoassay/ elisa kits
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Enzyme Immunoassay/ Elisa Kits, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/enzyme+immunoassay++elisa+kits/pm23794464-60-23-37
Average 90 stars, based on 1 article reviews
enzyme immunoassay/ elisa kits - by Bioz Stars, 2026-10
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enzyme immunoassay (elisa) commercial kits bd opteiatm  (Becton Dickinson)
90
Becton Dickinson enzyme immunoassay (elisa) commercial kits bd opteiatm
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Enzyme Immunoassay (Elisa) Commercial Kits Bd Opteiatm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/enzyme+immunoassay++elisa++commercial+kits+bd+opteiatm/pm37805488-99-5-11
Average 90 stars, based on 1 article reviews
enzyme immunoassay (elisa) commercial kits bd opteiatm - by Bioz Stars, 2026-10
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human enzyme-immunoassay elisa kits  (Mediagnost GmbH)
90
Mediagnost GmbH human enzyme-immunoassay elisa kits
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Human Enzyme Immunoassay Elisa Kits, supplied by Mediagnost GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/human+enzyme+immunoassay+elisa+kits/pmc05708702-140-8-7
Average 90 stars, based on 1 article reviews
human enzyme-immunoassay elisa kits - by Bioz Stars, 2026-10
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elisa immunoassays kits for testosterone and 17β-estradiol  (Abnova)
90
Abnova elisa immunoassays kits for testosterone and 17β-estradiol
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Elisa Immunoassays Kits For Testosterone And 17β Estradiol, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/elisa+immunoassays+kits+for+testosterone+and+17%CE%B2+estradiol/pmc04813352-108-9-10
Average 90 stars, based on 1 article reviews
elisa immunoassays kits for testosterone and 17β-estradiol - by Bioz Stars, 2026-10
90/100 stars
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quantitative sandwich enzyme immunoassays elisa kits  (Becton Dickinson)
90
Becton Dickinson quantitative sandwich enzyme immunoassays elisa kits
CD69 engagement <t>induces</t> <t>TGF-β1</t> secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by <t>ELISA.</t> Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).
Quantitative Sandwich Enzyme Immunoassays Elisa Kits, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunoassay+elisa+kits/quantitative+sandwich+enzyme+immunoassays+elisa+kits/pmc02719183-43-14-16
Average 90 stars, based on 1 article reviews
quantitative sandwich enzyme immunoassays elisa kits - by Bioz Stars, 2026-10
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Image Search Results


Monomeric bevacizumab significantly decreases when phosphate buffered saline (PBS) is used as a diluent. Representative SE-HPLC chromatograms showing bevacizumab in PBS at time 0 and after 3 h and graphical representation of SE-HPLC area and the slope for bevacizumab diluted with PBS at time 0 versus 3 h at room temperature ( a ), graphical representation of SE-HPLC area and the slope of the effect of dilution on monomer concentration of ranibizumab over time at 37°C in the manufacturer’s formulation; formula 1 ( b ). Shown are time 0 and after 24 h storage at 37°C.

Journal: Pharmaceutical Research

Article Title: Formulation Stabilization and Disaggregation of Bevacizumab, Ranibizumab and Aflibercept in Dilute Solutions

doi: 10.1007/s11095-018-2368-7

Figure Lengend Snippet: Monomeric bevacizumab significantly decreases when phosphate buffered saline (PBS) is used as a diluent. Representative SE-HPLC chromatograms showing bevacizumab in PBS at time 0 and after 3 h and graphical representation of SE-HPLC area and the slope for bevacizumab diluted with PBS at time 0 versus 3 h at room temperature ( a ), graphical representation of SE-HPLC area and the slope of the effect of dilution on monomer concentration of ranibizumab over time at 37°C in the manufacturer’s formulation; formula 1 ( b ). Shown are time 0 and after 24 h storage at 37°C.

Article Snippet: ELISA analytical kits for bevacizumab (kit #AVA-E-U51) and ranibizumab (kit #LUC-E-U52) were obtained from United Immunoassay Inc., San Bruno, CA USA.

Techniques: Saline, Concentration Assay, Formulation

Results of studies of bevacizumab, ranibizumab and aflibercept, diluted with PBS, and compared to dilutions with formula 14 at room temperature (23°C) ( a ). Correlation between enzyme-linked immunoassay (ELISA) and size exclusion high performance liquid chromatography (SE-HPLC) analytical methods ( b , c , d ). Figs. 6b, 6c and 6d provide graphical representation of bevacizumab, ranibizumab and aflibercept respectively. The data plots show the Pearson Moment Correlation coefficient r value.

Journal: Pharmaceutical Research

Article Title: Formulation Stabilization and Disaggregation of Bevacizumab, Ranibizumab and Aflibercept in Dilute Solutions

doi: 10.1007/s11095-018-2368-7

Figure Lengend Snippet: Results of studies of bevacizumab, ranibizumab and aflibercept, diluted with PBS, and compared to dilutions with formula 14 at room temperature (23°C) ( a ). Correlation between enzyme-linked immunoassay (ELISA) and size exclusion high performance liquid chromatography (SE-HPLC) analytical methods ( b , c , d ). Figs. 6b, 6c and 6d provide graphical representation of bevacizumab, ranibizumab and aflibercept respectively. The data plots show the Pearson Moment Correlation coefficient r value.

Article Snippet: ELISA analytical kits for bevacizumab (kit #AVA-E-U51) and ranibizumab (kit #LUC-E-U52) were obtained from United Immunoassay Inc., San Bruno, CA USA.

Techniques: Enzyme-linked Immunosorbent Assay, High Performance Liquid Chromatography

Results of Disaggregation Study Showing SE-HPLC Slopes of Bevacizumab, Ranibizumab and Aflibercept

Journal: Pharmaceutical Research

Article Title: Formulation Stabilization and Disaggregation of Bevacizumab, Ranibizumab and Aflibercept in Dilute Solutions

doi: 10.1007/s11095-018-2368-7

Figure Lengend Snippet: Results of Disaggregation Study Showing SE-HPLC Slopes of Bevacizumab, Ranibizumab and Aflibercept

Article Snippet: ELISA analytical kits for bevacizumab (kit #AVA-E-U51) and ranibizumab (kit #LUC-E-U52) were obtained from United Immunoassay Inc., San Bruno, CA USA.

Techniques: Control

Comparison of the two analytical methods ELISA ( a ) and SE-HPLC ( b ) for ranibizumab diluted with Formula 14 and PBS.

Journal: Pharmaceutical Research

Article Title: Formulation Stabilization and Disaggregation of Bevacizumab, Ranibizumab and Aflibercept in Dilute Solutions

doi: 10.1007/s11095-018-2368-7

Figure Lengend Snippet: Comparison of the two analytical methods ELISA ( a ) and SE-HPLC ( b ) for ranibizumab diluted with Formula 14 and PBS.

Article Snippet: ELISA analytical kits for bevacizumab (kit #AVA-E-U51) and ranibizumab (kit #LUC-E-U52) were obtained from United Immunoassay Inc., San Bruno, CA USA.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay

CD69 engagement induces TGF-β1 secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by ELISA. Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).

Journal: The Journal of Experimental Medicine

Article Title: Enhanced Antitumor Immunity in Mice Deficient in CD69

doi: 10.1084/jem.20021337

Figure Lengend Snippet: CD69 engagement induces TGF-β1 secretion in NK and CD3 + T lymphocytes. Antibody engagement of CD69 induces TGF-β1 secretion in mouse-purified NK and T lymphocytes. Anti-CD69 (CD69.2.2) or the isotypic control (murine IgG1) was added followed by cross-linking with goat anti–mouse IgG antibody. (A) Active (left) and total (right) TGF-β1 were determined in NK cells after 24 h in culture supernatants. (B) Purified resting CD3 + T cells were added to 96-well plates coated with anti-CD3. Expression of CD69 after activation with anti-CD3 were analyzed by staining with FITC–anti-murine CD69 mAb (shaded line) after 24 h of culture. Dotted line represents negative control. (C) Antibody engagement of CD69 induces TGF-β1 secretion in mouse T lymphocytes. Cells were incubated in serum-free medium for 72 h and TGF-β1 was determined by ELISA. Data are expressed as mean ± SD of replicate wells and are representative of three independent experiments. (D) Cultured cells were removed and trypan blue was added. Dead cells (trypan blue positive) was determined after 48 h of culture. Data represent means of triplicate wells (bars ± SD).

Article Snippet: Cultures were incubated (37°C, 5% CO 2 ), supernatants were collected after 24, 48, and 72 h, and secreted TGF-β was assayed with a TGF-β1 Emax ImmunoAssay System (Promega) ELISA kit, according to the manufacturer's protocol.

Techniques: Purification, Control, Expressing, Activation Assay, Staining, Negative Control, Incubation, Enzyme-linked Immunosorbent Assay, Cell Culture